Progress in precise and predictable genome editing in plants with base editing

Publication date: 19/04/2021
Extent: 24 pages

Contributions by: Sabine Fräbel, Shai J. Lawit, Jingyi Nie, David G. Schwark, Thomas J. Poorten and Nathaniel D. Graham, Pairwise Plants, USA

Chapter synopsis: Base editors are gene editing tools that allow targeted nucleic acid conversions, most commonly C>T and A>G, through pairing of deamination domains with impaired nucleases. Multiple deaminase domains and architectures have been demonstrated in planta across a wide array of species, with both cytosine and adenine base editing frequencies being observed at over 80%. The ability of base editors to introduce nucleic acid diversity while maintaining the same reading frame should make them powerful tools for plant genetic editing moving forward.

DOI: 10.19103/AS.2020.0082.06

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