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Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants

Code: 9781786768636
Publication date: 08/02/2021
Extent: 36 pages

Contributions by: William Gordon-Kamm, Pierluigi Barone, Sergei Svitashev, Jeffry D. Sander, Sandeep Kumar and Todd Jones, Corteva Agriscience, USA

Chapter synopsis: Despite the conspicuous and rapid development of genome editing tools, implementing this technology in plants often remains constrained by our inability to regenerate fertile genome-modified plants. Finding a viable combination of delivery method, genome editing reagents, and plant regeneration system has often been both crop-specific and crop-limited. Recent progress using morphogenic genes such as Wuschel2 (Wus2) and Babyboom (Bbm) has alleviated some of these crop-specific challenges, and along with other improvements, continues to expand the range of plants that can be edited.

DOI: 10.19103/AS.2020.0082.12

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Table of contents 1 Introduction 2 Delivery of genome editing components into plant cells 3 Delivery methods for genome editing reagents: delivery into single cells 4 Delivery methods for genome editing reagents: delivery into intact tissues 5 Alternatives to DNA delivery 6 Morphogenic genes increase transformation efficiency and extend genotype range 7 Morphogenic genes permit transformation in nontraditional explants 8 Future trends 9 Where to look for further information 10 References

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