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Advances in testing seed health

Code: 9781801465465
Publication date: 25/07/2022
Extent: 32 pages

Contributions by: Peter Bonants, Wageningen University & Research, The Netherlands

Chapter synopsis: Many different plant pathogens can be seed transmitted. In a world of global trade seeds infected with different plant pests are a potential risk. Seed health testing is important to prevent the spread of diseases. For the detection of these seed transmitted pests many methods are available e.g. ELISA, IF, dilution plating, PCR and real-time PCR. This chapter discusses several methods for seed health testing. It also evaluates the use of NGS sequencing for the detection of the most important seed transmitted pathogens in tomato: Clavibacter michiganensis subsp. michiganensis, Xanthomonas euvesicatoria, X. perforans, X. vesicatoria and X. gardneri, Pseudomonas syringae pv. tomato, Pepino Mosaic Virus and Potato spindle tuber viroid. These methods are compared with TaqMan PCR methods.

DOI: 10.19103/AS.2022.0105.10
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Table of contents 1 Introduction 2 Traditional detection methods 3 Serological detection techniques 4 Nucleic acid-based detection methods 5 Spectroscopy-based methods 6 Case study: detecting pathogens in tomato seeds 7 Summary and future trends 8 Where to look for further information 9 References

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