Advances in screening plants for edits and off-targets

Publication date: 19/04/2021
Extent: 16 pages

Contributions by: Chun Wang and Kejian Wang, China National Rice Research Institute, China

Chapter synopsis: Genome editing technology greatly accelerates the development of plant molecular research and crop breeding. However, accurately detecting mutations induced by sequence-specific nucleases between large-scale plants produced from transformation become a challenge. This chapter introduces some frequently-used molecular methods of identify genome editing induced mutation or off-targets, which developed in recent years. It also discusses the advantages and disadvantages of these methods and the scope of application, providing a useful reference for researchers who will identify genome-edited plants.

DOI: 10.19103/AS.2020.0082.14