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Mapping and exploiting the barley genome: techniques for mapping genes and relating them to desirable traits

Code: 9781786766083
Publication date: 03/02/2020
Extent: 34 pages

Contributions by: Hélène Pidon and Nils Stein, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany

Chapter synopsis: Barley is the fourth most cultivated cereal in the world and is thus an important crop in breeding. A large variety of traits are needed for resistance to various stresses as well as to meet the requirements of its several uses. To breed efficiently for those traits, identification of the causal genes and availability of genetic markers for selection are important targets for research. This chapter offers an overview of recent changes in this field, emphasizing the importance of the availability of barley reference genomes and sequencing technologies. The chapter outlines the methods and resources for mapping barley genes, covering developments over time and presenting a detailed case study. Finally, the chapter looks ahead to future research trends in this area and offers guidance on further reading.

DOI: 10.19103/AS.2019.0060.05
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Table of contents 1 Introduction 2 New possibilities for genetic mapping in the genomics era 3 Classical mapping strategies and their improvement in the genomics era 4 The association mapping boom 5 Multiparental populations: the perfect balance? 6 From an interval to the causal gene: from high-resolution mapping to gene cloning 7 Emerging mapping strategies: fast NGS-enabled technologies 8 Conservation of barley germplasm 9 Genetic and genomic resources of barley 10 Case study: from rym4 to rym11, illustration of paradigm shift in disease resistance mapping and cloning 11 Conclusion and future trends 12 Acknowledgement 13 Where to look for further information 14 References

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